(Additionally, the quality of slides is improved if they are stained in the field.) Slides should be placed in a protective holder (slide tray) and stored at room temperature. Slides should be air dried and fixed with slide fixative. The remainder of the blood should be spun at 3,500 rpm for 10 minutes and the separated serum decanted into cryotubes for long-term storage.

This blood will be used for packed cell volume (PCV), total solids (TS), white blood cell (WBC) counts, filter paper dot blots and blood smear slides (appendices 5 - 9). A small amount of whole blood (blood in anticoagulant) should be put into a few microcapillary tubes. Blood in both tubes should be centrifuged within 4 hours of collection to separate blood fractions.

Red top tubes should be kept at room temperature and purple top tubes are bested placed under refrigeration (i.e., cooler with ice). This will help to prevent the blood from clotting. Once blood is placed in purple top tubes, these tubes must be gently inverted a few times to mix the blood with the anticoagulant. Storage - Blood must be immediately transferred to red top tubes containing no anticoagulant and purple top tubes containing EDTA anticoagulant.

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